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簡要描述：SK-N-SH 人神經(jīng)母細胞瘤細胞原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和*培養(yǎng)條件！
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SK-N-SH 人神經(jīng)母細胞瘤細胞
| ATCC® Number: | HTB-11™ | Price: | $276.00 |
| Designations:SK-N-SHDepositors:G Trempe, LJ OldBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial SK-N-SH 人神經(jīng)母細胞瘤細胞Source:Organ: brain Disease: neuroblastoma Derived from metastatic site: bone marrowCellular Products:plasminogen activator shows increased expression of M-CSF after treatment with amyloid-beta peptidePermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.Applications:transfection host (Nucleofection technology from Lonza)Antigen Expression:Blood Type A; Rh+DNA Profile (STR):Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 8,13 D5S818: 12 D7S820: 7,10 THO1: 7,10 TPOX: 8,11 vWA: 14,18Cytogenetic Analysis:The cell line is hyperdiploid human female (XX), with the modal chromosome number of 47. Normal chromosomes N9 and N22 are single. One copy of each of these chromosomes is structurally altered to form the two marker chromosomes 9q+ and 22q+., Chromosomes N7 is trisomic. Extra bands were found on one copy of chromosome N7, thereby forming a marker chromosome as described by R.C. Seeger. May have been translocated in part(s) to the q arms of chromosomes N9 and N22.Isoenzymes:AK-1, 1 ES-D, 1G6PD, BGLO-I, 1 Me-2, 2 PGM1, 1 PGM3, 1Age:4 yearsGender:femaleComments:The SK-N-SH line was developed by J.L. Biedler and differs from SK-N-MC (see ATCC HTB-10) in that it exhibits a longer doubling time and higher levels of dopamine - beta - hydroxylase. SK-N-SH has been used as a target cell line in cell mediated cytotoxicity assays.PropagatRef | |||
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